A noncatalytic function of the topoisomerase II CTD in Aurora B recruitment to inner centromeres during mitosis.

Citation data:

The Journal of cell biology, ISSN: 1540-8140, Vol: 213, Issue: 6, Page: 651-64

Publication Year:
2016
Usage 18
Abstract Views 17
Link-outs 1
Captures 38
Readers 38
Social Media 9
Tweets 9
Citations 9
Citation Indexes 9
PMID:
27325791
DOI:
10.1083/jcb.201511080
PMCID:
PMC4915189
Author(s):
Edgerton, Heather; Johansson, Marnie; Keifenheim, Daniel; Mukherjee, Soumya; Chacón, Jeremy M; Bachant, Jeff; Gardner, Melissa K; Clarke, Duncan J
Publisher(s):
Rockefeller University Press
Tags:
Biochemistry, Genetics and Molecular Biology
Most Recent Tweet View All Tweets
article description
Faithful chromosome segregation depends on the precise timing of chromatid separation, which is enforced by checkpoint signals generated at kinetochores. Here, we provide evidence that the C-terminal domain (CTD) of DNA topoisomerase IIα (Topo II) provides a novel function at inner centromeres of kinetochores in mitosis. We find that the yeast CTD is required for recruitment of the tension checkpoint kinase Ipl1/Aurora B to inner centromeres in metaphase but is not required in interphase. Conserved CTD SUMOylation sites are required for Ipl1 recruitment. This inner-centromere CTD function is distinct from the catalytic activity of Topo II. Genetic and biochemical evidence suggests that Topo II recruits Ipl1 via the Haspin-histone H3 threonine 3 phosphorylation pathway. Finally, Topo II and Sgo1 are equally important for Ipl1 recruitment to inner centromeres. This indicates H3 T3-Phos/H2A T120-Phos is a universal epigenetic signature that defines the eukaryotic inner centromere and provides the binding site for Ipl1/Aurora B.