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Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov.

International Journal of Systematic and Evolutionary Microbiology, ISSN: 1466-5034, Vol: 72, Issue: 8
2022
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New Azospirillum brasilense Study Results Reported from National University Rio Cuarto (Genome-based Reclassification of Azospirillum Brasilense Az39 As the Type Strain of Azospirillum Argentinense Sp. Nov)

2023 JAN 02 (NewsRx) -- By a News Reporter-Staff News Editor at NewsRx Life Science Daily -- Fresh data on Gram-Negative Bacteria - Azospirillum brasilense

Article Description

Strain Az39 of Azospirillum is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3-acetic acid in the presence of l-tryptophan as a precursor, grows at 20–38 °C (optimal 38 °C), and the cells are curved or spiral-shaped, with diameters ranging from 0.5–0.9 to 1.8–2.2 µm. They contain C, C and C ω7c/ω6c as the main fatty acids. Phylogenetic analysis of its 16S rRNA gene sequence confirmed that this strain belongs to the genus Azospirillum, showing a close relationship with Azospirillum baldaniorum Sp245, Azospirillum brasilense Sp7 and Azospirillum formosense CC-Nfb-7. Housekeeping gene analysis revealed that Az39, together with five strains of the genus (Az19, REC3, BR 11975, MTCC4035 and MTCC4036), form a cluster apart from A. baldaniorum Sp245, A. brasilense Sp7 and A. formosense CC-Nfb-7. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Az39 and the aforementioned type strains revealed values below 96 %, the circumscription limit for the species delineation (ANI: 95.3, 94.1 and 94.0 %; dDDH: 62.9, 56.3 and 55.6 %). Furthermore, a phylogeny evaluation of the core proteome, including 809 common shared proteins, showed an independent grouping of Az39, Az19, REC3, BR 11975, MTCC4035 and MTCC4036. The G+C content in the genomic DNA of these six strains varied from 68.3 to 68.5 %. Based on the combined phylogenetic, genomic and phenotypic characterization presented here, we consider that strain Az39, along with strains Az19, REC3, BR 11975, MTCC4035 and MTCC4036, are members of a new Azospirillum species, for which the name Azospirillum argentinense sp. nov. is proposed. The type strain is Az39 (=LBPCV39=BR 148428=CCCT 22.01).

Bibliographic Details

Dos Santos Ferreira, Natália; Coniglio, Anahí; Puente, Mariana; Sant'Anna, Fernando Hayashi; Maroniche, Guillermo; García, Julia; Molina, Romina; Nievas, Sofia; Volpiano, Camila Gazolla; Ambrosini, Adriana; Passaglia, Luciane M P; Pedraza, Raul O; Reis, Verônica Massena; Zilli, Jerri Édson; Cassan, Fabricio

Microbiology Society

Immunology and Microbiology; Agricultural and Biological Sciences

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