PlumX Metrics
Embed PlumX Metrics

Massive sequencing of the V3-V4 hypervariable region of bronchoalveolar lavage from patients with COVID-19 and VAP reveals the collapse of the pulmonary microbiota

Journal of Medical Microbiology, ISSN: 1473-5644, Vol: 71, Issue: 12
2022
  • 7
    Citations
  • 0
    Usage
  • 17
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

Background. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is a predisposing factor for the development of healthcare-associated infections, of which ventilator-associated pneumonia (VAP) is one. Hypothesis. VAP is caused by ESKAPE bacteria and other pathogens not detected by microbiological culture. Aim. To elucidate the bacterial pathogens of severe coronavirus disease 2019 (COVID-19) and VAP patients by massive sequencing and to predict their degree of relationship with the age and sex of the patients. Methods. Analysis of ribosomal libraries of the V3-V4 hypervariable region obtained by Illumina sequencing of bronchoalveolar lavages from COVID-19 and VAP (first wave) patients from Hospital Juárez de México. Results. Acinetobacter and Pseudomonas were the main bacterial genera in the bronchoalveolar lavages (BALs) analysed. Other members of the ESKAPE group, such as Enterococcus and Klebsiella, were also identified. Taxonomic composition per patient showed that non-ESKAPE genera were present with significant relative abundances, such as Prevotella, Stenotrophomas, Enterococcus, Mycoplasma, Serratia and Corynebacterium. Kruskal-Wallis analysis proved that VAP acquisition is an adverse event that is not influenced by the sex and age of COVID-19 patients. Discussion. Metagenomic findings in COVID-19/VAP patients highlight the importance of implementing comprehensive microbiological diagnostics by including alternative tools for the detection of the causal agents of healthcare-associated infections (HAIs). Conclusions. Timely identification of bacteria 'not sought' in diagnostic bacteriology laboratories will allow specific and targeted treatments. Implications for the restricted diagnosis of VAP causative agents in COVID-19 patients and the presence of pathogens not detected by classical microbiology are analysed and discussed.

Bibliographic Details

Durán-Manuel, Emilio Mariano; Loyola-Cruz, Miguel Ángel; Cruz-Cruz, Clemente; Ibáñez-Cervantes, Gabriela; Gaytán-Cervantes, Javier; González-Torres, Carolina; Quiroga-Vargas, Edith; Calzada-Mendoza, Claudia Camelia; Cureño-Díaz, Monica Alethia; Fernández-Sánchez, Verónica; Castro-Escarpulli, Graciela; Bello-López, Juan Manuel

Microbiology Society

Immunology and Microbiology; Medicine

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know