Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
Protein Science, ISSN: 0961-8368, Vol: 14, Issue: 9, Page: 2236-2245
2005
- 16Citations
- 29Captures
- 1Mentions
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Metrics Details
- Citations16
- Citation Indexes16
- CrossRef16
- 16
- Captures29
- Readers29
- 29
- Mentions1
- News Mentions1
- News1
Article Description
The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (DVFSAVRADDSPTGEMQVAQY ) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins. Copyright © 2005 The Protein Society.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=24344462098&origin=inward; http://dx.doi.org/10.1110/ps.051526305; http://www.ncbi.nlm.nih.gov/pubmed/16131654; https://onlinelibrary.wiley.com/doi/10.1110/ps.051526305; https://dx.doi.org/10.1110/ps.051526305; https://onlinelibrary.wiley.com/doi/full/10.1110/ps.051526305; https://onlinelibrary.wiley.com/doi/abs/10.1110/ps.051526305; https://onlinelibrary.wiley.com/doi/pdf/10.1110/ps.051526305; http://doi.wiley.com/10.1110/ps.051526305; https://www.onlinelibrary.wiley.com/doi/abs/10.1110/ps.051526305; https://www.onlinelibrary.wiley.com/doi/full/10.1110/ps.051526305; https://www.onlinelibrary.wiley.com/doi/pdf/10.1110/ps.051526305; http://onlinelibrary.wiley.com/doi/10.1110/ps.051526305/abstract?systemMessage=Wiley+Online+Library+will+be+disrupted+on+16th+May+from+12%3A00-14%3A00+BST+%2807%3A00-09%3A00+EDT%29+for+up+to+two+hours+for+essential+maintenance.++Apologies+for+the+inconvenience.; http://onlinelibrary.wiley.com/doi/10.1110/ps.051526305/abstract
Wiley
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