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Gallic and carnosic acids improve quality of frozen-thawed ram spermatozoa

Andrologia, ISSN: 1439-0272, Vol: 51, Issue: 10, Page: e13393
2019
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Effect of Different Sonication Protocols on Merino Ram Sperm Parameters

Keywords: Sonication, Sperm viability, Methionine additive, Spermatozoa, TOS, TAS level, Ram serum, PNA-FITC staining INTRODUCTION Sperm freezing is an innovative assisted reproductive technology that enables

Article Description

The objective was to determine effects of gallic acid (GA) and carnosic acid (CA), present in carob pods and rosemary extract respectively, on frozen-thawed ram spermatozoa. Thirty ejaculates were collected from five Merino rams, pooled, diluted in Tris-based extender and divided into five equal portions containing: 0.05 or 2 mM of GA; 0.05 or 0.2 mM of CA; or no additive (control). Extended semen was equilibrated at +4°C, loaded into straws, held 5 cm above liquid nitrogen for 12 min then plunged. Computer-aided sperm analysis was used to assess motility, whereas flow cytometry was used to assess high mitochondrial membrane potential (HMMP) and percentages of spermatozoa with plasma membrane and acrosome integrity (PMAI). Spermatozoa supplemented with 2 mM GA had greater total motility than control spermatozoa (39.9 ± 3.01 vs. 29.2 ± 1.31%, mean ± SEM, p <.05). The PMAI was greatest in 0.2 mM CA (13.3 ± 0.68%), whereas HMMP was highest in 0.05 mM CA but lowest in control (22.9 ± 4.95 and 11.4 ± 3.64% respectively; p <.05). In conclusion, for cryopreservation of ram semen in Tris-based extender, supplementation with 2 mM GA increased post-thaw motility, whereas supplementation with 0.05 mM CA enhanced mitochondrial function.

Bibliographic Details

Gungor, Sukru; Inanc, Muhammed E; Ozturk, Caner; Korkmaz, Firat; Bastan, Ilktan; Cil, Beste; Kastelic, John P

Hindawi Limited

Biochemistry, Genetics and Molecular Biology; Medicine

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