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Red blood cell glutathione peroxidase activity in female nulligravid and pregnant rats

Reproductive Biology and Endocrinology, ISSN: 1477-7827, Vol: 7, Issue: 1, Page: 7
2009
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Background: The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise. Methods: Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes. Results: The optimization, when applied to age paired rats, both nulligravid and pregnant, shows that pregnancy induces, at seventeen d of pregnancy, an increase of both reactive oxygen substance concentration in red blood cells and membrane glutathione peroxidase activity. Conclusion: The glutathione peroxidase increase in erythrocyte membranes is induced by systemic oxidative stress long lasting rat pregnancy. © 2009 Gallo and Martino; licensee BioMed Central Ltd.

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