In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles
Parasites and Vectors, ISSN: 1756-3305, Vol: 9, Issue: 1, Page: 411
2016
- 28Citations
- 18Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations28
- Citation Indexes28
- 28
- CrossRef7
- Captures18
- Readers18
- 18
Article Description
Background: Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of larval E. multilocularis. Results: The protoscoleces (PSC) of E. multilocularis from jirds were successfully cultured in a modified RPMI1640 based medium containing 25 % (v/v) fetal bovine serum (FBS). After 100 days of culture, PSC developed to larval vesicles (small unilocular cysts) and the fast growing vesicles produced PSC in brood capsules. In addition, mice were intraperitoneally injected with 30 cultured small vesicles and 100 % of the mice had resulting metacestode masses. Conclusions: Larval protoscoleces and vesicles of E. multilocularis grow healthily in vitro in the RPMI1640 based medium containing 25 % FBS. Echinococcus multilocularis in vitro and in vivo models provide a valuable platform for investigating the biology of the parasite and screening effective therapeutic drugs against AE.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84979220409&origin=inward; http://dx.doi.org/10.1186/s13071-016-1687-y; http://www.ncbi.nlm.nih.gov/pubmed/27457380; http://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1687-y; https://dx.doi.org/10.1186/s13071-016-1687-y; https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1687-y
Springer Science and Business Media LLC
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