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Differential turnover rates of monocyte-derived cells in varied ocular tissue microenvironments

Journal of Leukocyte Biology, ISSN: 0741-5400, Vol: 84, Issue: 3, Page: 721-729
2008
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Article Description

Monocytes of bone marrow (BM) origin are circulating precursors that replenish dendritic cells and macrophage populations in peripheral tissues during homeostasis. The eye provides a unique range of varying tissue microenvironments in which to compare the different turnover rates of monocyte-derived cells. This was investigated in the present study using radiation chimeras, whereby BM from Cx3cr1 mice was used to rescue myeloablated wild-type (WT) BALB/c mice (conventional chimeras). The use of Cx3cr1 mice as BM donors allowed the clear visualization of newly recruited monocyte-derived cells. Following BM reconstitution, mice were killed at 2, 4, 6, and 8 weeks, and wholemount ocular tissues were processed for immunohistochemistry and confocal microscopy. "Reverse" chimeras (WT into Cx3cr1) were also created to act as a further method of crossreferencing cell turnover rates. In conventional chimeras, Cx3cr1 cells began repopulating the uveal tract (iris, ciliary body, choroid) 2 weeks posttransplantation with close to complete replenishment by 8 weeks. By contrast, the earliest recruitment of Cx3cr1 cells into the host retina occurred at 4 weeks. In reverse chimeras, a steady accumulation of host Cx3cr1 macrophages in the subretinal space of Cx3cr1 adult mice suggests that these cells arise from long-term resident microglia and not newly recruited WT donor cells. In summary, chimeric mouse models, in which lineage-specific cells carry a fluorescent reporter, have been used in the present study to visualize the turnover of monocyte-derived cells in different tissue compartments of the eye. These data provide valuable insights into differential monocyte turnover rates within a single complex organ. © Society for Leukocyte Biology.

Bibliographic Details

http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=50849130262&origin=inward; http://dx.doi.org/10.1189/jlb.0308166; http://www.ncbi.nlm.nih.gov/pubmed/18577714; https://academic.oup.com/jleukbio/article/84/3/721/6975182; http://www.jleukbio.org/cgi/doi/10.1189/jlb.0308166; http://www.jleukbio.org/content/84/3/721.short; https://research-repository.uwa.edu.au/en/publications/differential-turnover-rates-of-monocytederived-cells-in-varied-ocular-tissue-microenvironments(9ec4e864-c97d-4290-af86-bf007ff5ccab).html; https://research-repository.uwa.edu.au/en/publications/differential-turnover-rates-of-monocyte-derived-cells-in-varied-o; http://www.jleukbio.org/content/84/3/721.abstract; http://www.jleukbio.org/content/84/3/721.full; http://www.jleukbio.org/content/84/3/721.full.pdf; http://www.jleukbio.org/content/84/3/721; https://research-repository.uwa.edu.au/en/publications/9ec4e864-c97d-4290-af86-bf007ff5ccab; http://www.jleukbio.org/lookup/doi/10.1189/jlb.0308166; https://jlb.onlinelibrary.wiley.com/doi/abs/10.1189/jlb.0308166; https://jlb.onlinelibrary.wiley.com/doi/full/10.1189/jlb.0308166; https://jlb.onlinelibrary.wiley.com/doi/pdf/10.1189/jlb.0308166; https://academic.oup.com/jleukbio/article-pdf/84/3/721/48540107/jlb0721.pdf; http://research-repository.uwa.edu.au/en/publications/differential-turnover-rates-of-monocytederived-cells-in-varied-ocular-tissue-microenvironments(9ec4e864-c97d-4290-af86-bf007ff5ccab).html; http://www.jleukbio.org/cgi/content/short/84/3/721; https://jlb.onlinelibrary.wiley.com/doi/10.1189/jlb.0308166

Kezic, Jelena; McMenamin, Paul G

Oxford University Press (OUP)

Medicine; Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology

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