Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)
Optics Express, ISSN: 1094-4087, Vol: 20, Issue: 24, Page: 26681-26695
2012
- 65Citations
- 98Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations65
- Citation Indexes65
- 65
- CrossRef59
- Captures98
- Readers98
- 95
Conference Paper Description
Super-resolution imaging with photo-activatable or photoswitchable probes is a promising tool in biological applications to reveal previously unresolved intra-cellular details with visible light. This field benefits from developments in the areas of molecular probes, optical systems, and computational post-processing of the data. The joint design of optics and reconstruction processes using double-helix point spread functions (DH-PSF) provides high resolution three-dimensional (3D) imaging over a long depth-of-field. We demonstrate for the first time a method integrating a Fisher information efficient DH-PSF design, a surface relief optical phase mask, and an optimal 3D localization estimator. 3D super-resolution imaging using photo-switchable dyes reveals the 3D microtubule network in mammalian cells with localization precision approaching the information theoretical limit over a depth of 1.2 μm. © 2012 Optical Society of America.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84870601655&origin=inward; http://dx.doi.org/10.1364/oe.20.026681; http://www.ncbi.nlm.nih.gov/pubmed/23187521; https://www.osapublishing.org/oe/abstract.cfm?uri=oe-20-24-26681&tab=supplmat; http://dx.doi.org/10.1364/oe.20.026681.m001; https://www.osapublishing.org/abstract.cfm?URI=oe-20-24-26681; https://www.osapublishing.org/viewmedia.cfm?URI=oe-20-24-26681&seq=0; https://opg.optica.org/abstract.cfm?URI=oe-20-24-26681; https://opg.optica.org/oe/abstract.cfm?uri=oe-20-24-26681&tab=supplmat; http://dx.doi.org/10.1364/oe.20.026681.m002; https://dx.doi.org/10.1364/oe.20.026681.m002; https://dx.doi.org/10.1364/oe.20.026681.m001; https://dx.doi.org/10.1364/oe.20.026681; https://opg.optica.org/oe/abstract.cfm?uri=oe-20-24-26681; https://opg.optica.org/abstract.cfm?uri=oe-20-24-26681; https://opg.optica.org/viewmedia.cfm?uri=oe-20-24-26681&seq=0&html=true; https://opg.optica.org/viewmedia.cfm?uri=oe-20-24-26681&seq=0; http://www.opticsinfobase.org/abstract.cfm?URI=oe-20-24-26681; https://www.osapublishing.org/abstract.cfm?uri=oe-20-24-26681; https://www.osapublishing.org/viewmedia.cfm?uri=oe-20-24-26681&seq=0&html=true; https://www.osapublishing.org/viewmedia.cfm?uri=oe-20-24-26681&seq=0; https://www.osapublishing.org/oe/abstract.cfm?uri=oe-20-24-26681; https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-20-24-26681&id=246022
The Optical Society
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