The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium
PLoS ONE, ISSN: 1932-6203, Vol: 13, Issue: 3, Page: e0193601
2018
- 127Citations
- 541Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations127
- Citation Indexes127
- 127
- CrossRef30
- Captures541
- Readers541
- 541
Article Description
THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The results show that THP-1 macrophages differentiated in high concentrations of PMA rapidly died following infection whereas those differentiated in low concentrations of PMA survived and were able to control the intracellular bacteria similar to primary human macrophages. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bibliographic Details
10.1371/journal.pone.0193601; 10.1371/journal.pone.0193601.g002; 10.1371/journal.pone.0193601.g003; 10.1371/journal.pone.0193601.g004; 10.1371/journal.pone.0193601.g001; 10.1371/journal.pone.0193601.g005; 10.1371/journal.pone.0193601.g006
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85043779505&origin=inward; http://dx.doi.org/10.1371/journal.pone.0193601; http://www.ncbi.nlm.nih.gov/pubmed/29538403; https://dx.plos.org/10.1371/journal.pone.0193601.g002; http://dx.doi.org/10.1371/journal.pone.0193601.g002; https://dx.plos.org/10.1371/journal.pone.0193601.g003; http://dx.doi.org/10.1371/journal.pone.0193601.g003; https://dx.plos.org/10.1371/journal.pone.0193601.g004; http://dx.doi.org/10.1371/journal.pone.0193601.g004; https://dx.plos.org/10.1371/journal.pone.0193601.g001; http://dx.doi.org/10.1371/journal.pone.0193601.g001; https://dx.plos.org/10.1371/journal.pone.0193601.g005; http://dx.doi.org/10.1371/journal.pone.0193601.g005; https://dx.plos.org/10.1371/journal.pone.0193601.g006; http://dx.doi.org/10.1371/journal.pone.0193601.g006; https://dx.plos.org/10.1371/journal.pone.0193601; https://dx.doi.org/10.1371/journal.pone.0193601.g001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g001; https://dx.doi.org/10.1371/journal.pone.0193601.g002; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g002; https://dx.doi.org/10.1371/journal.pone.0193601.g003; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g003; https://dx.doi.org/10.1371/journal.pone.0193601; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0193601; https://dx.doi.org/10.1371/journal.pone.0193601.g005; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g005; https://dx.doi.org/10.1371/journal.pone.0193601.g004; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g004; https://dx.doi.org/10.1371/journal.pone.0193601.g006; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g006; http://dx.plos.org/10.1371/journal.pone.0193601; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0193601&type=printable; http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0193601
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