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The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium

PLoS ONE, ISSN: 1932-6203, Vol: 13, Issue: 3, Page: e0193601
2018
  • 127
    Citations
  • 0
    Usage
  • 541
    Captures
  • 0
    Mentions
  • 33
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    127
  • Captures
    541
  • Social Media
    33
    • Shares, Likes & Comments
      33
      • Facebook
        33

Article Description

THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The results show that THP-1 macrophages differentiated in high concentrations of PMA rapidly died following infection whereas those differentiated in low concentrations of PMA survived and were able to control the intracellular bacteria similar to primary human macrophages. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85043779505&origin=inward; http://dx.doi.org/10.1371/journal.pone.0193601; http://www.ncbi.nlm.nih.gov/pubmed/29538403; https://dx.plos.org/10.1371/journal.pone.0193601.g002; http://dx.doi.org/10.1371/journal.pone.0193601.g002; https://dx.plos.org/10.1371/journal.pone.0193601.g003; http://dx.doi.org/10.1371/journal.pone.0193601.g003; https://dx.plos.org/10.1371/journal.pone.0193601.g004; http://dx.doi.org/10.1371/journal.pone.0193601.g004; https://dx.plos.org/10.1371/journal.pone.0193601.g001; http://dx.doi.org/10.1371/journal.pone.0193601.g001; https://dx.plos.org/10.1371/journal.pone.0193601.g005; http://dx.doi.org/10.1371/journal.pone.0193601.g005; https://dx.plos.org/10.1371/journal.pone.0193601.g006; http://dx.doi.org/10.1371/journal.pone.0193601.g006; https://dx.plos.org/10.1371/journal.pone.0193601; https://dx.doi.org/10.1371/journal.pone.0193601.g001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g001; https://dx.doi.org/10.1371/journal.pone.0193601.g002; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g002; https://dx.doi.org/10.1371/journal.pone.0193601.g003; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g003; https://dx.doi.org/10.1371/journal.pone.0193601; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0193601; https://dx.doi.org/10.1371/journal.pone.0193601.g005; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g005; https://dx.doi.org/10.1371/journal.pone.0193601.g004; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g004; https://dx.doi.org/10.1371/journal.pone.0193601.g006; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0193601.g006; http://dx.plos.org/10.1371/journal.pone.0193601; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0193601&type=printable; http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0193601

Tregei Starr; Timothy J. Bauler; Preeti Malik-Kale; Olivia Steele-Mortimer; Dennis C. Ko

Public Library of Science (PLoS)

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Multidisciplinary

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