Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.

Citation data:

PloS one, ISSN: 1932-6203, Vol: 12, Issue: 1, Page: e0171171

Publication Year:
2017
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PMID:
28125737
DOI:
10.1371/journal.pone.0171171.g005, 10.1371/journal.pone.0171171.g004, 10.1371/journal.pone.0171171.g006, 10.1371/journal.pone.0171171.g001, 10.1371/journal.pone.0171171.g003, 10.1371/journal.pone.0171171.g002, 10.1371/journal.pone.0171171
Author(s):
Michael C. Rahe, Michael P. Murtaugh, Gourapura J. Renukaradhya
Publisher(s):
Public Library of Science (PLoS), Figshare
Tags:
Medicine, Biochemistry, Genetics and Molecular Biology, Agricultural and Biological Sciences, Cell Biology, Physiology, Biotechnology, Immunology, Biological Sciences not elsewhere classified, Developmental Biology, Cancer, Hematology, Infectious Diseases, Virology, surface immunoglobulin receptor, 40L, IL -21, memory cell differentiation, Antibody Secreting Cells Immunological prevention, Interleukin -21 Drives Proliferation, IgG antibody secreting cells, Porcine Memory B Cells, ïve age-matched pigs, B cells, Mature B cells, BAFF, ELISA, memory B cell culture system, IL -21-driven expansion, ASC, PRRSV non-structural protein 7 ELISPOT, memory B cells, 69999 Biological Sciences not elsewhere classified, 40l, il -21, antibody secreting cells immunological prevention, interleukin -21 drives proliferation, igg antibody secreting cells, porcine memory b cells, b cells, mature b cells, baff, elisa, memory b cell culture system, il -21-driven expansion, asc, prrsv non-structural protein 7 elispot, memory b cells
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article description
Immunological prevention of infectious disease, especially viral, is based on antigen-specific long-lived memory B cells. To test for cellular proliferation and differentiation factors in swine, an outbred model for humans, CD21+ B cells were activated in vitro with CD40L and stimulated with purported stimulatory cytokines to characterize functional responses. IL-21 induced a 3-fold expansion in total cell numbers with roughly 15% of all B cells differentiating to IgM or IgG antibody secreting cells (ASCs.) However, even with robust proliferation, cellular viability rapidly deteriorated. Therefore, a proliferation inducing ligand (APRIL) and B cell activating factor (BAFF) were evaluated as survival and maintenance factors. BAFF was effective at enhancing the viability of mature B cells as well as ASCs, while APRIL was only effective for ASCs. Both cytokines increased approximately two-fold the amount of IgM and IgG which was secreted by IL-21 differentiated ASCs. Mature B cells from porcine reproductive and respiratory virus (PRRSV) immune and naïve age-matched pigs were activated and treated with IL-21 and then tested for memory cell differentiation using a PRRSV non-structural protein 7 ELISPOT and ELISA. PRRSV immune pigs were positive on both ELISPOT and ELISA while naïve animals were negative on both assays. These results highlight the IL-21-driven expansion and differentiation of memory B cells in vitro without stimulation of the surface immunoglobulin receptor complex, as well as the establishment of a defined memory B cell culture system for characterization of vaccine responses in outbred animals.

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