Pax7 remodels the chromatin landscape in skeletal muscle stem cells.

Citation data:

PloS one, ISSN: 1932-6203, Vol: 12, Issue: 4, Page: e0176190

Publication Year:
2017
Usage 2882
Full Text Views 2777
Views 44
Abstract Views 39
Downloads 21
Link-outs 1
Captures 32
Readers 32
Social Media 12
Tweets 11
Shares, Likes & Comments 1
PMID:
28441415
DOI:
10.1371/journal.pone.0176190
Author(s):
Karin C. Lilja, Nan Zhang, Alessandro Magli, Volkan Gunduz, Christopher J. Bowman, Robert W. Arpke, Radbod Darabi, Michael Kyba, Rita Perlingeiro, Brian D. Dynlacht, Atsushi Asakura Show More Hide
Publisher(s):
Public Library of Science (PLoS), Figshare
Tags:
Biochemistry, Genetics and Molecular Biology, Agricultural and Biological Sciences, Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Physiology, Developmental Biology, Cancer, Infectious Diseases, Space Science, chromatin accessibility, pax 7, pax 7 binding, pax 7 remodels, myogenic differentiation genes, psc, myogenic precursors pre-figures, muscle cells, muscle precursor cells, h 3k tri-methylated regions, satellite cell specification, pax 7 binding sites, chromatin state, satellite cell compartment
Most Recent Tweet View All Tweets
article media
article description
Pluripotent stem cells (PSC) hold great promise for the treatment of human skeletal muscle diseases. However, it remains challenging to convert PSC to skeletal muscle cells, and the mechanisms by which the master regulatory transcription factor, Pax7, promotes muscle stem (satellite) cell identity are not yet understood. We have taken advantage of PSC-derived skeletal muscle precursor cells (iPax7), wherein the induced expression of Pax7 robustly initiates the muscle program and enables the in vitro generation of precursors that seed the satellite cell compartment upon transplantation. Remarkably, we found that chromatin accessibility in myogenic precursors pre-figures subsequent activation of myogenic differentiation genes. We also found that Pax7 binding is generally restricted to euchromatic regions and excluded from H3K27 tri-methylated regions in muscle cells, suggesting that recruitment of this factor is circumscribed by chromatin state. Further, we show that Pax7 binding induces dramatic, localized remodeling of chromatin characterized by the acquisition of histone marks associated with enhancer activity and induction of chromatin accessibility in both muscle precursors and lineage-committed myoblasts. Conversely, removal of Pax7 leads to rapid reversal of these features on a subset of enhancers. Interestingly, another cluster of Pax7 binding sites is associated with a durably accessible and remodeled chromatin state after removal of Pax7, and persistent enhancer accessibility is associated with subsequent, proximal binding by the muscle regulatory factors, MyoD1 and myogenin. Our studies provide new insights into the epigenetic landscape of skeletal muscle stem cells and precursors and the role of Pax7 in satellite cell specification.

This article has 0 Wikipedia mention.