Acridine orange fluorescent microscopy is more sensitive than India ink light microscopy in the rapid detection of cryptococcosis among CrAg positive HIV patients.

Citation data:

PloS one, ISSN: 1932-6203, Vol: 12, Issue: 7, Page: e0182108

Publication Year:
2017
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PMID:
28750078
DOI:
10.1371/journal.pone.0182108
Author(s):
Richard Kwizera, Andrew Akampurira, Darlisha Williams, David R. Boulware, David B. Meya, On Behalf Of The Astro Cm Study Team
Publisher(s):
Public Library of Science (PLoS)
Tags:
Biochemistry, Genetics and Molecular Biology, Agricultural and Biological Sciences
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article description
India ink microscopy on cerebrospinal fluid is still utilized in resource limited settings for the diagnosis of cryptococcal meningitis despite its poor sensitivity. We hypothesized that staining fungal nucleic acids with fluorescent dyes instead of the capsule with India ink might improve sensitivity for the diagnosis of cryptococcal meningitis.

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