Reversal of the anticoagulant and anti-hemostatic effect of low molecular weight heparin by direct prothrombin activation
Brazilian Journal of Medical and Biological Research, ISSN: 1414-431X, Vol: 45, Issue: 10, Page: 929-934
2012
- 3Citations
- 6,916Usage
- 20Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations3
- Citation Indexes3
- CrossRef3
- Scielo1
- Usage6,916
- Full Text Views6,394
- 6,394
- Abstract Views522
- 522
- Captures20
- Readers20
- 20
Article Description
Lopap, found in the bristles of Lonomia obliqua caterpillar, is the first exogenous prothrombin activator that shows serine protease-like activity, independent of prothrombinase components and unique lipocalin reported to interfere with hemostasis mechanisms. To assess the action of an exogenous prothrombin activator reversing the anticoagulant and antihemostatic effect induced by low molecular weight heparin (LMWH), male New Zealand rabbits (N = 20, weighing 3.8-4.0 kg) allocated to 4 groups were anticoagulated with 1800 IU/kg LMWH (iv) over 2 min, followed by iv administration of saline or recombinant Lopap (rLopap) at 1 μg/kg (LG1) or 10 μg/kg (LG10), 10 min after the injection of LMWH, in a blind manner. Control animals were treated only with saline. The action of rLopap was assessed in terms of activated partial thromboplastin time (aPTT), prothrombin fragment F1+2, fibrinogen, and ear puncture bleeding time (BT) at 5, 10, 15, 17, 20, 30, 40, 60, and 90 min after initiation of LMWH infusion. LG10 animals showed a decrease of aPTT in more than 50% and BT near to normal baseline. The level of prothrombin fragment F1+2 measured by ELISA had a 6-fold increase with rLopap treatment (10 μg/kg) and was inversely proportional to BT in LMWH-treated animals. Thus, Lopap, obtained in recombinant form using E. coli expression system, was useful in antagonizing the effect of LMWH through direct prothrombin activation, which can be a possible strategy for the reversal of bleeding and anticoagulant events.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84866028513&origin=inward; http://dx.doi.org/10.1590/s0100-879x2012007500108; http://www.ncbi.nlm.nih.gov/pubmed/22735179; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000007&lng=en&tlng=en; http://www.scielo.br/pdf/bjmbr/v45n10/1971.pdf; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012001000007&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000007; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012001000007; https://dx.doi.org/10.1590/s0100-879x2012007500108; https://www.scielo.br/j/bjmbr/a/6hCZTNZQ4QSnZcgqnVtzFQD/?lang=en
FapUNIFESP (SciELO)
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know