Oral bioavailability of glyphosate: Studies using two intestinal cell lines
Environmental Toxicology and Chemistry, ISSN: 0730-7268, Vol: 24, Issue: 1, Page: 153-160
2005
- 32Citations
- 50Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations32
- Citation Indexes28
- 28
- CrossRef25
- Policy Citations4
- Policy Citation4
- Captures50
- Readers50
- 50
Article Description
Glyphosate is a commonly used nonselective herbicide that inhibits plant growth through interference with the production of essential aromatic amino acids. In vivo studies in mammals with radiolabeled glyphosate have shown that 34% of radioactivity was associated with intestinal tissue 2 h after oral administration. The aim of our research was to investigate the transport, binding, and toxicity of glyphosate to the cultured human intestinal epithelial cell line, Caco-2, and the rat small intestinal crypt-derived cell line, ileum epithelial cells-18 (IEC-18). An in vitro analysis of the transport kinetics of [C]-glyphosate showed that 4 h after exposure, approximately 8% of radiolabeled glyphosate moved through the Caco-2 monolayer in a dose-dependent manner. Binding of glyphosate to cells was saturable and approximately 4 × 10 binding sites/cell were estimated from bound [C]. Exposure of Caco-2 cells to ≥10 mg/ml glyphosate reduced transmembrane electrical resistance (TEER) by 82 to 96% and increased permeability to [ H]-mannitol, indicating that paracellular permeability increased in glyphosate-treated cells. At 10-mg/ml glyphosate, both IEC-18 and Caco-2 cells showed disruption in the actin cytoskeleton. In Caco-2 cells, significant lactate dehydrogenase leakage was observed when cells were exposed to 15 mg/ml of glyphosate. These data indicate that at doses >10 mg/ml, glyphosate significantly disrupts the barrier properties of cultured intestinal cells.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10644252854&origin=inward; http://dx.doi.org/10.1897/04-088r.1; http://www.ncbi.nlm.nih.gov/pubmed/15683179; https://academic.oup.com/etc/article/24/1/153/7770342; http://doi.wiley.com/10.1897/04-088R.1; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1897%2F04-088R.1; https://dx.doi.org/10.1897/04-088r.1; https://setac.onlinelibrary.wiley.com/doi/abs/10.1897/04-088R.1
Oxford University Press (OUP)
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