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A Surface-Enhanced Raman Scattering  Aptasensor for Escherichia Coli Detection Based on High-Performance 3D Substrate And Hot Spot Effect

SSRN Electronic Journal
  • 1
    Citations
  • 184
    Usage
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    Captures
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Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    1
    • Citation Indexes
      1
  • Usage
    184
    • Abstract Views
      165
    • Downloads
      19

Article Description

A surface-enhanced Raman scattering (SERS) aptasensor was established for highly sensitive and specific detection of Escherichia coli (E. coli), which based on high-performance SERS 3D substrate and hot spot effect. Chitosan hydrogel (CS gel) modified with E. coli aptamer (Apt) functionalized silver nanoparticles (Apt-AgNPs-CS gel) were constructed as a high-performance SERS 3D substrate for specific bacteria enrichment, while the Raman signaling molecule 4-MBA and E. coli Apt modified gold nanostar (GNS@4-MBA-Apt NPs) were synthesized based on hot spot effect for the sensitive quantification of E. coli. Due to the huge SERS enhancement based on hot spot effect and the specific recognition ability of Apt, a detection limit of 3.46 CFU/mL with a wide dynamic linear range from 3.2×101 to 3.2×107 CFU/mL (R2 =0.99875) could be achieved without other non-target bacteria interference. Moreover, this SERS aptasensor was applied to detect E. coli in actual samples with good recovery rate. In addition, this SERS strategy can be extended to the detection of other targets by replacing the corresponding Apts. Hence our SERS aptasensor has potential application in food safety and environmental pollution monitoring.

Bibliographic Details

Yufei Ye; Xiaoyu Qi; Hao Wang; Bianbian Zhao; Lixia Xu; Yuting Zhang; Xiaoli Wang; Nandi Zhou

Elsevier BV

Escherichia coli; Surface-enhanced Raman scattering; Aptasensor; SERS 3D substrate; hot spot effect

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