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Fenton Reaction Enhanced Enzyme Free Electrochemical Biosensor for Rapid and Highly Sensitive Detection of Salmonella

SSRN, ISSN: 1556-5068
2024
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Article Description

To fulfill the stringent demands of clinical Salmonella detection, this study employed the benefits of Ni-TCPP(Fe2+) and ConA to establish a novel approach that is culture-free, enzyme-free, secondary antibody-free, and ultra-sensitive. Our investigations revealed two key mechanisms that significantly bolster the signal-to-noise ratio and enhance detection stability: firstly, the Fenton reaction and the mimic enzyme catalysis of Ni-TCPP(Fe2+) improve the sensitivity of the biosensor; secondly, the substitution of the secondary antibody with ConA leads to increased binding of Ni-TCPP(Fe2+) due to the abundance of ConA receptors on the bacterial surface. With proper assembly, the developed biosensor exhibited high sensitivity in the range of 101–107 CFU/mL, along with a detection limit as low as 4 CFU/mL, and achieved rapid detection within a mere 2 hours. Moreover, this biosensor has proven its efficacy in detecting Salmonella in clinical biological samples. Its advantages of being culture-free, secondary antibody-free, and ultra-sensitive establish a solid research foundation for the expedited detection of Salmonella in clinical settings and point-of-care testing (POCT).

Bibliographic Details

Ying Zheng; Qing Li; Zhongzhu Yang; Ruyi Yang; Xin Liu; Shijia Ding; Daobin Han

Elsevier BV

Multidisciplinary; Fenton reaction; Ni-TCPP(Fe2+); ConA; electrochemical biosensor; Salmonella

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