Fenton Reaction Enhanced Enzyme Free Electrochemical Biosensor for Rapid and Highly Sensitive Detection of Salmonella
SSRN, ISSN: 1556-5068
2024
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
To fulfill the stringent demands of clinical Salmonella detection, this study employed the benefits of Ni-TCPP(Fe2+) and ConA to establish a novel approach that is culture-free, enzyme-free, secondary antibody-free, and ultra-sensitive. Our investigations revealed two key mechanisms that significantly bolster the signal-to-noise ratio and enhance detection stability: firstly, the Fenton reaction and the mimic enzyme catalysis of Ni-TCPP(Fe2+) improve the sensitivity of the biosensor; secondly, the substitution of the secondary antibody with ConA leads to increased binding of Ni-TCPP(Fe2+) due to the abundance of ConA receptors on the bacterial surface. With proper assembly, the developed biosensor exhibited high sensitivity in the range of 101–107 CFU/mL, along with a detection limit as low as 4 CFU/mL, and achieved rapid detection within a mere 2 hours. Moreover, this biosensor has proven its efficacy in detecting Salmonella in clinical biological samples. Its advantages of being culture-free, secondary antibody-free, and ultra-sensitive establish a solid research foundation for the expedited detection of Salmonella in clinical settings and point-of-care testing (POCT).
Bibliographic Details
Elsevier BV
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