Measuring neuromuscular junction functionality
Journal of Visualized Experiments, ISSN: 1940-087X, Vol: 2017, Issue: 126
2017
- 6Citations
- 35Captures
- 1Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations6
- Citation Indexes6
- CrossRef5
- Captures35
- Readers35
- 35
- Mentions1
- News Mentions1
- News1
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Measuring Neuromuscular Junction Functionality.
J Vis Exp. 2017 Aug 6;(126) Authors: Rizzuto E, Pisu S, Nicoletti C, Del Prete Z, Musarò A PubMed: 28809841 Submit Comment
Article Description
Neuromuscular junction (NMJ) functionality plays a pivotal role when studying diseases in which the communication between motor neuron and muscle is impaired, such as aging and amyotrophic lateral sclerosis (ALS). Here we describe an experimental protocol that can be used to measure NMJ functionality by combining two types of electrical stimulation: direct muscle membrane stimulation and the stimulation through the nerve. The comparison of the muscle response to these two different stimulations can help to define, at the functional level, potential alterations in the NMJ that lead to functional decline in muscle. Ex vivo preparations are suited to well-controlled studies. Here we describe an intensive protocol to measure several parameters of muscle and NMJ functionality for the soleus-sciatic nerve preparation and for the diaphragm-phrenic nerve preparation. The protocol lasts approximately 60 min and is conducted uninterruptedly by means of a custom-made software that measures the twitch kinetics properties, the force-frequency relationship for both muscle and nerve stimulations, and two parameters specific to NMJ functionality, i.e. neurotransmission failure and intratetanic fatigue. This methodology was used to detect damages in soleus and diaphragm muscle-nerve preparations by using SOD1G93A transgenic mouse, an experimental model of ALS that ubiquitously overexpresses the mutant antioxidant enzyme superoxide dismutase 1 (SOD1).
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85027067457&origin=inward; http://dx.doi.org/10.3791/55227; http://www.ncbi.nlm.nih.gov/pubmed/28809841; https://app.jove.com/t/55227; https://dx.doi.org/10.3791/55227; https://www.jove.com/t/55227/measuring-neuromuscular-junction-functionality
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