Using RAPD and ISSR markers to assess genetic diversity and conservation of Bruguiera gymnorrhiza (L.) Lam. for sustainable management on Kerala's West Coast
Journal of Applied Biology and Biotechnology, ISSN: 2347-212X, Vol: 12, Issue: 6, Page: 105-111
2024
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Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
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Article Description
In this genetic study focusing on Kerala’s west coast, particularly the southern region with diverse demographics and fluctuating salinity, we utilized random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) molecular markers to assess the genetic variation of the plant species Bruguiera gymnorrhiza, which is facing habitat loss in the region. The ISSR marker demonstrated a higher degree of polymorphism (13.6%) compared with RAPD (3.5%). The polymorphic information content averaged 0.484 for ISSR and 0.354 for RAPD. The B. gymnorrhiza samples from Alappuzha and Thrissur clustered together in both markers. Nei’s genetic diversity index (h) was determined to be 0.430 for RAPD and 0.453 for ISSR. The unweighted pair group with the arithmetic mean dendrogram, reflecting a greater degree of polymorphism, supports the conservation of B. gymnorrhiza throughout Kerala’s west coast. Additionally, the marker index was calculated as 0.506 for ISSR and 0.392 for RAPD, highlighting the efficacy of ISSR markers in assessing the genetic diversity of B. gymnorrhiza.
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