FlpStop, a tool for conditional gene control in .

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eLife, ISSN: 2050-084X, Vol: 6

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10.7554/elife.22279; 10.7554/elife.22279.021; 10.7554/elife.22279.009; 10.7554/elife.22279.014; 10.7554/elife.22279.001; 10.7554/elife.22279.016; 10.7554/elife.22279.005; 10.7554/elife.22279.022; 10.7554/elife.22279.017; 10.7554/elife.22279.013; 10.7554/elife.22279.008; 10.3410/f.727314991.793536256; 10.7554/elife.22279.002
Fisher, Yvette E; Yang, Helen H; Isaacman-Beck, Jesse; Xie, Marjorie; Gohl, Daryl M; Clandinin, Thomas R
eLife Sciences Organisation, Ltd.; eLife Sciences Publications, Ltd; Faculty of 1000, Ltd.
Neuroscience; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
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article description
Manipulating gene function cell type-specifically is a common experimental goal in research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cell division. Here we describe FlpStop, a generalizable tool for conditional gene disruption and rescue in post-mitotic cells. In proof-of-principle experiments, we manipulated , a regulator of wing development. Next, we produced conditional null alleles of () and (), genes vital for GABAergic neurotransmission, as well as () and (), voltage-gated ion channels central to neuronal excitability. To demonstrate the utility of this approach, we manipulated in a specific visual interneuron type and discovered differential regulation of calcium signals across subcellular compartments. Thus, FlpStop will facilitate investigations into the interactions between genes, circuits, and computation.