PlumX Metrics
Embed PlumX Metrics

Immunofluorometric quantitation and histochemical localisation of kallikrein 6 protein in ovarian cancer tissue: A new independent unfavourable prognostic biomarker

British Journal of Cancer, ISSN: 0007-0920, Vol: 87, Issue: 7, Page: 763-771
2002
  • 71
    Citations
  • 0
    Usage
  • 27
    Captures
  • 2
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    71
  • Captures
    27
  • Mentions
    2
    • References
      2
      • 2

Article Description

Human kallikrein 6 protein is a newly discovered human kallikrein. We determined the amount of human kallikrein 6 in extracts of 182 ovarian tumours and correlated specific activity (ng hK6 mg total protein) with clinicopathological variables documented at the time of surgical excision and with outcome (progression free survival, overall survival) monitored over a median interval of 62 months. Thirty per cent of the tumours were positive for human kallikrein 6 (>35 ng hK6 mg total protein). Human kallikrein 6-specific immunohistochemical staining of four ovarian tissues that included benign, borderline and malignant lesions indicated a cytoplasmic location of human kallikrein 6 in tumour cells of epithelial origin, although the intensity of staining was variable. Tumour human kallikrein 6 (ng hK6 mg total protein) was higher in late stage disease, serous histotype, residual tumour > 1 cm and suboptimal debulking (> 1 cm) (P<0.05), Univariate analysis revealed that patients with tumour human kallikrein 6 positive specific activity were more likely to suffer progressive disease and to die (hazard ratio 1.71 (P=0.015) and 1.88 (P=0.022), respectively). Survival curves demonstrated the same (P=0.013 and 0.019, respectively). Multivariate analysis revealed that human kallikrein 6 positivity was retained as an independent prognostic variable in several subgroups of patients, namely those with (low) grade I and II tumours (hazard ratio progression free survival 4.3 (P=0.027) and overall survival 4.1 (P=0.023)) and those with optimal debulking (hazard ratio progression free survival 3.8 (P=0.019) and overall survival 5.6 (P=0.011)). We conclude that tumour kallikrein 6 protein levels have utility as an independent adverse prognostic marker in a subgroup of ovarian cancer patients with otherwise apparently good prognosis. © 2002 Cancer Research UK.

Bibliographic Details

B. R. Hoffman; P. Diamandis; T. Colgan; E. P. Diamandis; D. Katsaros; S. Fracchioli; I. A. Rigault de la Longrais; M. Puopolo; M. Massobrio; A. Scorilas; G. Giardina

Springer Science and Business Media LLC

Medicine; Biochemistry, Genetics and Molecular Biology

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know