Identification and purification of the aspartate/glutamate carrier from bovine heart mitochondria
Biochimica et Biophysica Acta (BBA) - Biomembranes, ISSN: 0005-2736, Vol: 1106, Issue: 2, Page: 291-296
1992
- 21Citations
- 11Captures
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Metrics Details
- Citations21
- Citation Indexes21
- 21
- CrossRef16
- Captures11
- Readers11
- 11
Article Description
The aspartate/glutamate carrier from bovine heart mitochondria was solubilized with dodecyl-octaoxyethylene ether (C 12 E 8 ) and purified by chromatography on hydroxyapatite and celite. On SDS gel electrophoresis, the purified aspartate/glutamate carrier consisted of a single protein band with an apparent M r of 31 500. When reconstituted into lipososomes the aspartate/glutamate carrier protein catalyzed an N -ethylmaleimide-sensitive aspartate/aspartate exchange. It was purified 620-fold with a recovery of 17.2% and a protein yield of 0.03% with respect to the mitochondrial extract. The properties of the reconstituted carrier, i.e. requirement for a counteranion, substrate specificity and inhibitor sensitivity, were similar to those of the aspartate/glutamate carrier as characterized in mitochondria.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/000527369290008A; http://dx.doi.org/10.1016/0005-2736(92)90008-a; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026535996&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/1317723; https://linkinghub.elsevier.com/retrieve/pii/000527369290008A; http://linkinghub.elsevier.com/retrieve/pii/000527369290008A; http://api.elsevier.com/content/article/PII:000527369290008A?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:000527369290008A?httpAccept=text/plain; http://dx.doi.org/10.1016/0005-2736%2892%2990008-a; https://dx.doi.org/10.1016/0005-2736%2892%2990008-a
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