Monitoring the temporal and spatial distribution of RNA in living yeast cells
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 419, Page: 187-196
2008
- 1Citations
- 21Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations1
- Citation Indexes1
- Captures21
- Readers21
- 21
Book Chapter Description
RNA localization is a cellular process to spatially restrict translation of specific proteins to defined regions within or between cells. Most localized mRNAs contain cis-acting localization elements in the 3′-untranslated region (UTR), which are sufficient for localization of an mRNA to a particular region of the cell. The cis-acting localization elements serve as assembly sites for trans-acting factors which function to sort the mRNA to the correct sub-cellular destination. Although fluorescent in situ hybridization (FISH) has been widely used to study mRNA localization, FISH has a weakness in that it is a static assay, as FISH requires that cells be fixed before hybridization. Consequently, FISH is not ideally suited for investigating dynamic mRNA localization processes. This limitation of FISH has been overcome by the development of techniques that allow the visualization of mRNA in living cells. Here, we present a protocol that tethers green fluorescent protein (GFP) to an mRNA of interest, allowing for the visualization of dynamic mRNA localization processes in living cells. © Humana Press.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=42949178004&origin=inward; http://dx.doi.org/10.1007/978-1-59745-033-1_13; http://www.ncbi.nlm.nih.gov/pubmed/18369984; http://link.springer.com/10.1007/978-1-59745-033-1_13; http://www.springerlink.com/index/10.1007/978-1-59745-033-1_13; http://www.springerlink.com/index/pdf/10.1007/978-1-59745-033-1_13; https://dx.doi.org/10.1007/978-1-59745-033-1_13; https://link.springer.com/protocol/10.1007/978-1-59745-033-1_13
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