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Altered ventilatory and thermoregulatory control in male and female adult Pet-1 null mice

Respiratory Physiology & Neurobiology, ISSN: 1569-9048, Vol: 177, Issue: 2, Page: 133-140
2011
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The integrity of the serotonin (5-HT) system is essential to normal respiratory and thermoregulatory control. Male and female transgenic mice lacking central 5-HT neurons ( Lmx1b f/f/p mice) show a 50% reduction in the hypercapnic ventilatory response and insufficient heat generation when cooled ( Hodges and Richerson, 2008a; Hodges et al., 2008b ). Lmx1b f/f/p mice also show reduced body temperatures ( T body ) and O 2 consumption V˙O2, and breathe less at rest and during hypoxia and hypercapnia when measured below thermoneutrality (24 °C), suggesting a role for 5-HT neurons in integrating ventilatory, thermal and metabolic control. Here, the hypothesis that Pet-1 null mice, which retain 30% of central 5-HT neurons, will demonstrate similar deficits in temperature and ventilatory control was tested. Pet-1 null mice had fewer medullary tryptophan hydroxylase-immunoreactive (TPH + ) neurons compared to wild type (WT) mice, particularly in the midline raphé. Female (but not male) Pet-1 null mice had lower baseline ventilation (V˙E), breathing frequency ( f ), V˙O2 and T body relative to female WT mice ( P < 0.05). In addition, V˙E and V˙E/V˙O2 were decreased in male and female Pet-1 null mice during hypoxia and hypercapnia ( P < 0.05), but only male Pet-1 null mice showed a significant deficit in the hypercapnic ventilatory response when expressed as % of control ( P < 0.05). Finally, male and female Pet-1 null mice showed significant decreases in T body when externally cooled to 4 °C. These data demonstrate that a moderate loss of 5-HT neurons leads to a modest attenuation of mechanisms defending body temperature, and that there are gender differences in the contributions of 5-HT neurons to ventilatory and thermoregulatory control.

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