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Lignin Peroxidase Oxidation of Mn in the Presence of Veratryl Alcohol, Malonic or Oxalic Acid, and Oxygen

Biochemistry, ISSN: 1520-4995, Vol: 29, Issue: 46, Page: 10475-10480
1990
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Veratryl alcohol (3,4-dimethoxybenzyl alcohol) appears to have multiple roles in lignin degradation by Phanerochaete chrysosporium. It is synthesized de novo by the fungus. It apparently induces expression of lignin peroxidase (LiP), and it protects LiP from inactivation by HO. In addition, veratryl alcohol has been shown to potentiate LiP oxidation of compounds that are not good LiP substrates. We have now observed the formation of Mn in reaction mixtures containing LiP, Mn, veratryl alcohol, maionate buffer, HO, and O. No Mn was formed if veratryl alcohol or HO was omitted. Mn formation also showed an absolute requirement for oxygen, and oxygen consumption was observed in the reactions. This suggests involvement of active oxygen species. In experiments using oxalate (a metabolite of P. chrysosporium) instead of maionate, similar results were obtained. However, in this case, we detected (by ESR spin-trapping) the production of carbon dioxide anion radical (CO) and perhydroxyl radical (OOH) in reaction mixtures containing LiP, oxalate, veratryl alcohol, HO, and O. Our data indicate the formation of oxalate radical, which decays to CO and CO The latter reacts with O to form O, which then oxidizes Mn to Mn. No radicals were detected in the absence of veratryl alcohol. These results indicate that LiP can indirectly oxidize Mn and that veratryl alcohol is probably a radical mediator in this system. © 1990, American Chemical Society. All rights reserved.

Bibliographic Details

Popp, Janet L.; Kalyanaraman, B.; Kirk, T. Kent

American Chemical Society (ACS)

Biochemistry, Genetics and Molecular Biology

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