On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation
Free Radical Biology and Medicine, ISSN: 0891-5849, Vol: 65, Page: 1310-1314
2013
- 112Citations
- 103Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations112
- Citation Indexes112
- 112
- CrossRef38
- Captures103
- Readers103
- 103
Article Description
L-012, a luminol-based chemiluminescent (CL) probe, is widely used in vitro and in vivo to detect NADPH oxidase (Nox)-derived superoxide (O 2 − ) and identify Nox inhibitors. Yet understanding of the free radical chemistry of the L-012 probe is still lacking. We report that peroxidase and H 2 O 2 induce superoxide dismutase (SOD)-sensitive, L-012-derived CL in the presence of oxygen. O 2 − alone does not react with L-012 to emit luminescence. Self-generated O 2 − during oxidation of L-012 and luminol analogs artifactually induce CL inhibitable by SOD. These aspects make assays based on luminol analogs less than ideal for specific detection and identification of O 2 − and NOX inhibitors.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0891584913006205; http://dx.doi.org/10.1016/j.freeradbiomed.2013.09.017; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84886048542&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/24080119; https://linkinghub.elsevier.com/retrieve/pii/S0891584913006205
Elsevier BV
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