Oxidation of ethidium-based probes by biological radicals: mechanism, kinetics and implications for the detection of superoxide
Scientific Reports, ISSN: 2045-2322, Vol: 10, Issue: 1, Page: 18626
2020
- 17Citations
- 18Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations17
- Citation Indexes17
- 17
- CrossRef5
- Captures18
- Readers18
- 18
Article Description
Hydroethidine (HE) and hydropropidine (HPr ) are fluorogenic probes used for the detection of the intra- and extracellular superoxide radical anion (O2∙-). In this study, we provide evidence that HE and HPr react rapidly with the biologically relevant radicals, including the hydroxyl radical, peroxyl radicals, the trioxidocarbonate radical anion, nitrogen dioxide, and the glutathionyl radical, via one-electron oxidation, forming the corresponding radical cations. At physiological pH, the radical cations of the probes react rapidly with O2∙-, leading to the specific 2-hydroxylated cationic products. We determined the rate constants of the reaction between O2∙- and the radical cations of the probes. We also synthesized N-methylated analogs of HPr and HE which were used in mechanistic studies. Methylation of the amine groups was not found to prevent the reaction between the radical cation of the probe and the superoxide, but it significantly increased the lifetime of the radical cation and had a substantial effect on the profiles of the oxidation products by inhibiting the formation of dimeric products. We conclude that the N-methylated analogs of HE and HPr may be used as a scaffold for the design of a new generation of probes for intra- and extracellular superoxide.
Bibliographic Details
Springer Science and Business Media LLC
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