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Antigen-specific detection of soluble immune complexes in conglutinin-binding assays

Clinical and Experimental Immunology, ISSN: 0009-9104, Vol: 45, Issue: 2, Page: 283-289
1981
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Article Description

A modification of the conglutinin-binding assay has been used for antigen identification in circulating immune complexes (IC). The complexed antigen was identified, in conglutinin-bound complexes, by antibodies or antibody fragments, specifically directed against the antigen. These antibody preparations were either radiolabelled or detected with I-protein A. This system was found efficient using in vitro-formed bovine serum albumin-anti-bovine serum albumin and tetanus toxoid-anti-tetanus toxoid soluble immune complexes at equivalence or at slight antigen or antibody excess. In addition, with I-IgG-anti-HBs and with I-F(ab')-anti-HBs, we examined 44 sera from 41 patients with viral type B hepatitis and the presence of HBs was observed in 18 (40.9%) IC containing tested samples. Therefore, this method appears applicable to clinical investigation.

Bibliographic Details

R. D'Amelio; G. Brighouse; M. Barnet; P. H. Lambert

Medicine; Immunology and Microbiology

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