Simultaneous Determination of Key Osmoregulants in Halophytes Using HPLC–ELSD

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Chromatographia, ISSN: 0009-5893, Vol: 76, Issue: 17-18, Page: 1125-1130

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Kalsoom, Umme; Boyce, Mary C; Bennett, Ian J; Veraplakorn, Varaporn
Springer Nature; Springer-Verlag
Chemistry; Biochemistry, Genetics and Molecular Biology; [RSTDPub]; Betaine; Column liquid chromatography; ELSD; Mannitol; Osmoregulators; Prolineacetonitrile; alcohol; Atriplex cinerea extract; betaine; formic acid; mannitol; plant extract; proline; Rhagodia baccata extract; Stylosanthes guianensis extract; unclassified drug; article; evaporative light scattering detection; halophyte; high performance liquid chromatography; light scattering; limit of detection; nonhuman; osmoregulation; priority journal; reliability; sensitivity analysis; Plant Biology
article description
Osmoregulants are the substances produced by plants that assist in tolerating environmental stresses. Three commonly analysed osomoregulants include mannitol, betaine and proline. A simple, sensitive and rapid HPLC-ELSD method has been developed for the simultaneous analysis of these common osmoregulants in plant extracts. Osmoregulants were extracted using 80 % ethanol and separated on an NHcolumn using 0.1 % formic acid and acetonitrile as the mobile phase. Retention time repeatability was 0.85, 1.50, and 0.93 % for mannitol, betaine and proline, respectively. The limit of detection (μmol) was 1.43 × 10, 7.81 × 10and 1.08 × 10for mannitol, betaine and proline, respectively. The developed method was applied to three different plant extracts, Stylosanthes guianensis, Atriplex cinerea and Rhagodia baccata. A second method using a C18 column with 0.1 % heptafluorobutyric acid and acetonitrile as the mobile phase proved to be a useful complementary method for verifying tentative peak identifications. © 2013 Springer-Verlag Berlin Heidelberg.