Validation of an automated colony counting system for group A Streptococcus.

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BMC research notes, ISSN: 1756-0500, Vol: 9, Issue: 1, Page: 72

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Frost, H R; Tsoi, S K; Baker, C A; Laho, D; Sanderson-Smith, Martina L; Steer, Andrew C; Smeesters, Pierre R
Springer Nature
Biochemistry, Genetics and Molecular Biology; Medicine and Health Sciences
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article description
The practice of counting bacterial colony forming units on agar plates has long been used as a method to estimate the concentration of live bacteria in culture. However, due to the laborious and potentially error prone nature of this measurement technique, an alternative method is desirable. Recent technologic advancements have facilitated the development of automated colony counting systems, which reduce errors introduced during the manual counting process and recording of information. An additional benefit is the significant reduction in time taken to analyse colony counting data. Whilst automated counting procedures have been validated for a number of microorganisms, the process has not been successful for all bacteria due to the requirement for a relatively high contrast between bacterial colonies and growth medium. The purpose of this study was to validate an automated counting system for use with group A Streptococcus (GAS).