Determination of total iron binding capacity of serum by capillary electrophoresis

Citation data:

Chromatographia, ISSN: 0009-5893, Vol: 49, Issue: 1-2, Page: 71-74

Publication Year:
1999
Usage 5
Abstract Views 5
Captures 3
Readers 3
Citations 4
Citation Indexes 4
Repository URL:
http://scholarsmine.mst.edu/chem_facwork/653
DOI:
10.1007/bf02467190
Author(s):
Ding, Yongsheng; Liu, L. L.; Ma, Yinfa; Lin, Bingcheng
Publisher(s):
Springer Nature; Springer Verlag
Tags:
Chemistry; Biochemistry, Genetics and Molecular Biology; Iron; Accuracy; Capillary Electrophoresis; Chemical Analysis; Iron Blood Level; Metal Binding; Priority Journal; Serum Analysis; Total Iron Binding Capacity; Iron; Accuracy; Capillary Electrophoresis; Chemical Analysis; Iron Blood Level; Metal Binding; Priority Journal; Serum Analysis; Total Iron Binding Capacity
article description
A capillary electrophoresis (CE) technique for determining total iron binding capacity (TIBC) of serum has been developed. The optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with Fe; then, dissociate them completely after removing excess unbound Fe. Finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the CE analysis. Ammonium acetate (pH = 5.0) was used as CE background electrolyte solution. In this system, a good linear correlation coefficient was maintained over the range 0.5~10 μM (r = 0.9979, n=12). Seven adult serum samples were studied and the TIBC parameters measured. In the present system, 10~30 μL serum is sufficient for determination. The study shows that the CE technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.