Intravital Microscopy of Tumor Oxygenation and Glycolytic Demand

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Ivers, Jesse D
Bioimaging and Biomedical Optics; Biological Engineering; Biomedical Devices and Instrumentation; Biomedical Engineering and Bioengineering
thesis / dissertation description
There is growing concern about the over treatment of cancer because treatments are based primarily on tumor anatomy. In this study, we aim to begin the process of addressing that issue by developing an intravital technique for optically analyzing tumor biology. Two traits were identified as having significant importance in the aggression of a tumor, vascular oxygenation (SO2) and glycolytic demand. Dorsal skin flap window chambers were implanted and 4T1 and 67NR cancer cells were injected to provide a tumor model for the development of this intravital quantification technique. This study provides a detailed protocol from instrumentation setup to surgical procedures to imaging methods as well as showing that optical techniques can be used to quantify SO2 and glycolytic demand within various cell lines.