Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome.

Citation data:

Biotechnology for biofuels, ISSN: 1754-6834, Vol: 9, Issue: 1, Page: 194

Publication Year:
2016
Usage 78
Abstract Views 47
Full Text Views 26
Downloads 5
Captures 32
Readers 32
Citations 6
Citation Indexes 6
Repository URL:
https://trace.tennessee.edu/utk_chembiopubs/99
PMID:
27602057
DOI:
10.1186/s13068-016-0607-x
PMCID:
PMC5012057
Author(s):
Thompson, R. Adam; Dahal, Sanjeev; Garcia, Sergio; Nookaew, Intawat; Trinh, Cong T
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Energy; Environmental Science; Clostridium thermocellum; Genome-scale model; Consolidated bioprocessing; Biofuels; Bioenergetics; Elementary mode analysis; Flux balance analysis; Rational strain design; Minimal cut sets; Chemical Engineering
article description
Clostridium thermocellum is a gram-positive thermophile that can directly convert lignocellulosic material into biofuels. The metabolism of C. thermocellum contains many branches and redundancies which limit biofuel production, and typical genetic techniques are time-consuming. Further, the genome sequence of a genetically tractable strain C. thermocellum DSM 1313 has been recently sequenced and annotated. Therefore, developing a comprehensive, predictive, genome-scale metabolic model of DSM 1313 is desired for elucidating its complex phenotypes and facilitating model-guided metabolic engineering.