Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome.
- Citation data:
Biotechnology for biofuels, ISSN: 1754-6834, Vol: 9, Issue: 1, Page: 194
- Publication Year:
- Repository URL:
- Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Energy; Environmental Science; Clostridium thermocellum; Genome-scale model; Consolidated bioprocessing; Biofuels; Bioenergetics; Elementary mode analysis; Flux balance analysis; Rational strain design; Minimal cut sets; Chemical Engineering
Clostridium thermocellum is a gram-positive thermophile that can directly convert lignocellulosic material into biofuels. The metabolism of C. thermocellum contains many branches and redundancies which limit biofuel production, and typical genetic techniques are time-consuming. Further, the genome sequence of a genetically tractable strain C. thermocellum DSM 1313 has been recently sequenced and annotated. Therefore, developing a comprehensive, predictive, genome-scale metabolic model of DSM 1313 is desired for elucidating its complex phenotypes and facilitating model-guided metabolic engineering.