A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings.
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Biotechnology for biofuels, ISSN: 1754-6834, Vol: 3, Issue: 1, Page: 9
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- Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Energy; Environmental Science; Plant Sciences
Grasses are relatively recalcitrant to genetic transformation in comparison to certain dicotyledons, yet they constitute some of the most important biofuel crops. Genetic transformation of switchgrass (Panicum virgatum L.) has previously been reported after cocultivation of explants with Agrobacterium and biolistics of embryogenic calli. Experiments to increase transient gene expression in planta may lead to stable transformation methods with increased efficiency.