An efficient microcarrier based Adeno-associated virus production method

Adeno-associated viruses (AAV) are reported to have a great potential for gene therapy. However, a major bottleneck for these kinds of therapies are efficient methods for viral production.The purpose of the present study was to explore how a production-cell culture attached to microcarriers in a stirred tank bioreactor could provide a solution for efficient AAV production. Microcarriers can provide an accelerated process development platform where an already high product yielding adherent cell clone can be directly transitioned to suspension production. Higher cell specific AAV yields are often reported for adherent cell systems compared to cells in suspension, and microcarrier-based culturing is well established for anchored cells in larger scale. Taken together, using microcarriers as an AAV production platform has the potential to increase cell specific yields and increase volumetric productivity thus lowering the total cost of goods for AAV based therapies.Please click Additional Files below to see the full abstract.