Mrub_0860, Mrub_0701 and Mrub_2285 are orthologous to E. coli b2892, b2562 and b3863 within the RecFOR pathway for Homologous Recombination

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tool associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict the gene function. We investigated the biological function of the genes Mrub_0860, Mrub_0701,and Mrub_2285. We predicted that Mrub_0860 (DNA coordinates 842934..844868 on the forward strand) encodes for the enzyme single-stranded DNA-specific exonuclease, which is in the first step of homologous recombination via the RecFOR pathway (KEGG map number 03440). The E. coli K12 MG1655 ortholog is predicted to be b2892, which has the gene identifier RecJ. It is predicted that Mrub_0701 (DNA coordinates 675799..676503 on the reverse strand) encodes for the recombinational DNA repair protein, which is in the second step, filament formation, of homologous recombination via the RecFOR pathway (KEGG map number 03440). The E. coli K12 MG1655 ortholog is predicted to be b2562, which has the gene identifier RecO. It is predicted that Mrub_2285 (DNA coordinates 2337447..2341268 on the reverse strand) encodes for the enzyme DNA polymerase I, which is the third step, DNA synthesis and strand invasion, of homologous recombination via the RecFOR pathway (KEGG map number 03440). The E. coli K12 MG1655 ortholog is predicted to be b3863, which has the gene identifier DpoI.