Synthesis and evaluation of the rhodamine- and biotin- probes for detection of cysteine containing proteins

Along with glutathione, biological thiol cysteine (Cys) plays important roles in many biological processes such as cellular detoxification and metabolism in living organisms. Changes in intracellular Cys concentration have a profound effect on these biological processes. Given the critical importance of Cys homeostasis in biological systems, a strong interest has emerged in developing effective probes to detect and image Cys-rich proteins. In this oral presentation, the synthesis and evaluation of probes which we are working on and would be used to label Cys-rich proteins in biological systems, especially in redox systems, will be presented.