Session 3F: The Difference between PTEN, EC-Confetti, and STIM1 Mice through Genotyping

With molecular biology starting to focus more on specific mechanisms and mutations, different types of mice are being generated. The question, thus arises, how to differentiate between these mice. Previous studies indicate the mechanisms behind breeding and creating certain specific- gene targeting -mice. However, there are few cost effective methods that are available in order to check whether mice have or lack certain genes. In this investigation I examined 3 different mice. This research was significant because PTEN mice, EC-Confetti, and STIM1 mice, and creating variations of them with Cre, can help people to better understand vascular biology. With the tails of the mice, I isolated the DNA and ran PCR and electrophoresis. The agarose blot provided us with information about the mouse- whether it was homozygous or heterozygous – which is important for us to differentiate between mice and find lineage. Further studies should be continued to learn more about other EC specific genes, and their genotyping over generations.