Identification and characterization of Tdd-1 and related retrotransposons in Dictyostelium.
1993
- 43Usage
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Usage43
- Downloads38
- Abstract Views5
Thesis / Dissertation Description
Previous studies in Dictyostelium discoideum on the transposable element Tdd-1, have identified two transcription units of opposite polarity. One is a major, developmentally regulated 4.5 kb transcript believed to encode proteins involved in the retrotransposition of this element, and the other, 1.4 kb in size, is a heat-shock inducible RNA. Based on the sequence of the 4.5 kb transcript and the encoded amino acid sequence, it has been proposed that Tdd-1 is a retrotransposon. This implies that the 4.5 kb mRNA is copied by reverse transcriptase, resulting in DNA copies that insert into new sites. The purpose of this study was to identify similar retrotransposons in two other Dictyostelium strains, D. discoideum V-12 and D. mucoroides DM-7, and to estimate retrotransposon copy number. To identify the Tdd-1 gene in D. discoideum strain NC-4, polymerase chain reaction (PCR) primers were designed using the published nucleotide sequence in the known highly conserved amino acid regions of reverse transcriptase. The product of this amplification was a 370-bp fragment, which was then cloned. The identity of the fragment as part of Tdd-1 was confirmed by restriction mapping and DNA sequencing. To estimate gene copy number, Southern blots were analyzed using a probe made from the cloned fragment. In all Southern blot analysis NC-4 had the highest copy number, followed by D. discoideum V-12 and D. mucoroides DM-7, respectively. Although the band patterns on Southern blots were not reproducible, D. discoideum NC-4 always had more bands than D. discoideum V-12, which had more bands than D. mucoroides DM-7. This was consistent with PCR results where NC-4 always yielded the greatest concentration of amplified product of the expected size. A probe was used to identify clones with complementary sequences to the Tdd-1 fragment in a genomic library of D. mucoroides. Digoxigenin (dig)-labeling of the cloned probe was successfully accomplished by employing the PCR. Identification of genomic clones from the lambda library was accomplished using these dig-labeled probes.
Bibliographic Details
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know