THE ROLE OF OXYGEN AS A REGULATOR OF NODAL EXPRESSION IN POORLY METASTATIC BREAST CANCER CELLS
2010
- 12Usage
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Usage12
- Downloads11
- Abstract Views1
Thesis / Dissertation Description
Recently, the stem cell factor Nodal has been correlated with metastatic competence in breast cancer. We investigated hypoxia as a regulator of Nodal as it is an important feature of stem and cancer cell microenvironments. We hypothesized that hypoxia up-regulates Nodal in poorly metastatic T47D breast cancer cells through the Hypoxia-Inducible Factor-1 (HIF-1) and Notch signalling pathways. We exposed cells to decreasing concentrations of O2 for varying amounts of time. 0.5% O2 for 48 h experienced a maximal induction of Nodal mRNA expression and protein levels, which persisted for up to 72 h with reoxygenation. HIF-lα over-expression and knock down experiments implicate a role for HIF-lα transcriptional regulation of Nodal. Dual luciferase assays, which investigated the Notch-responsive enhancer region (NDE) upstream of Nodal, showed activation in hypoxia. Mutations in the NDE abrogated this activity. This work reveals how a normally stem cell-associated factor can be up- regulated by hypoxia in cancer cells.
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