Fusion activity and inactivation of influenza virus: Kinetics of low pH-induced fusion with cultured cells
Journal of General Virology, ISSN: 0022-1317, Vol: 73, Issue: 1, Page: 27-37
1992
- 42Citations
- 41Usage
- 22Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations42
- Citation Indexes42
- 42
- CrossRef35
- Usage41
- Downloads36
- Abstract Views5
- Captures22
- Readers22
- 22
Article Description
The kinetics of fusion of influenza virus (A/PR/8/34) with human promyelocytic leukaemia (HL-60), human T lymphocytic leukaemia (CEM) and murine lymphoma (S49) cells were investigated. Fusion was demonstrated by electron microscopy, and monitored by fluorescence dequenching of octadecylrhodamine incorporated in the virus membrane. Rapid fusion was induced upon mild acidification of the medium. At pH 5, all virus particles were capable of fusing with the cells. The initial rate and the extent of fusion were maximal between pH 4.9 and 5.2 and declined sharply below and above this range. The rate constants of adhesion of influenza virus to cells or erythrocyte ghosts were large, indicating a diffusion-controlled process. The rate constants of fusion of the virus with cells were smaller than those found previously for fusion with various liposomes. Although preincubation of the virus at acidic pH in the absence of target membranes almost completely inactivated the virus in its ability to fuse with erythrocyte ghosts, it reduced the extent of fusion with cultured cells by only 20 to 40%. Kinetic analysis of fusion revealed a mode of inactivation of the virus bound to erythrocyte ghosts or suspension cells, below pH 5.4, different from that of the virus preincubated at low pH without target membranes.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026504652&origin=inward; http://dx.doi.org/10.1099/0022-1317-73-1-27; http://www.ncbi.nlm.nih.gov/pubmed/1730942; https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-1-27; https://scholarlycommons.pacific.edu/dugoni-facarticles/656; https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?article=1655&context=dugoni-facarticles; https://dx.doi.org/10.1099/0022-1317-73-1-27
Microbiology Society
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