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Dissecting biological "dark matter" with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth

Proceedings of the National Academy of Sciences of the United States of America, ISSN: 0027-8424, Vol: 104, Issue: 29, Page: 11889-11894
2007
  • 515
    Citations
  • 426
    Usage
  • 635
    Captures
  • 8
    Mentions
  • 1
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    515
  • Usage
    426
  • Captures
    635
  • Mentions
    8
    • References
      7
      • Wikipedia
        7
    • News Mentions
      1
      • News
        1
  • Social Media
    1
    • Shares, Likes & Comments
      1
      • Facebook
        1

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Article Description

We have developed a microfluidic device that allows the isolation and genome amplification of individual microbial cells, thereby enabling organism-level genomic analysis of complex microbial ecosystems without the need for culture. This device was used to perform a directed survey of the human subgingival crevice and to isolate bacteria having rod-like morphology. Several isolated microbes had a 16S rRNA sequence that placed them in candidate phylum TM7, which has no cultivated or sequenced members. Genome amplification from individual TM7 cells allowed us to sequence and assemble >1,000 genes, providing insight into the physiology of members of this phylum. This approach enables single-cell genetic analysis of any uncultivated minority member of a microbial community. © 2007 by The National Academy of Sciences of the USA.

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