Dissecting biological "dark matter" with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth
Proceedings of the National Academy of Sciences of the United States of America, ISSN: 0027-8424, Vol: 104, Issue: 29, Page: 11889-11894
2007
- 515Citations
- 426Usage
- 635Captures
- 8Mentions
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations515
- Citation Indexes511
- 511
- CrossRef455
- Patent Family Citations4
- Patent Families4
- Usage426
- Downloads355
- Abstract Views71
- Captures635
- Readers635
- 635
- Mentions8
- References7
- Wikipedia7
- News Mentions1
- News1
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Article Description
We have developed a microfluidic device that allows the isolation and genome amplification of individual microbial cells, thereby enabling organism-level genomic analysis of complex microbial ecosystems without the need for culture. This device was used to perform a directed survey of the human subgingival crevice and to isolate bacteria having rod-like morphology. Several isolated microbes had a 16S rRNA sequence that placed them in candidate phylum TM7, which has no cultivated or sequenced members. Genome amplification from individual TM7 cells allowed us to sequence and assemble >1,000 genes, providing insight into the physiology of members of this phylum. This approach enables single-cell genetic analysis of any uncultivated minority member of a microbial community. © 2007 by The National Academy of Sciences of the USA.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34547540925&origin=inward; http://dx.doi.org/10.1073/pnas.0704662104; http://www.ncbi.nlm.nih.gov/pubmed/17620602; https://pnas.org/doi/full/10.1073/pnas.0704662104; https://scholarworks.sjsu.edu/biol_pub/5; https://scholarworks.sjsu.edu/cgi/viewcontent.cgi?article=1003&context=biol_pub; https://dx.doi.org/10.1073/pnas.0704662104; https://www.pnas.org/content/104/29/11889; http://f1000.com/1088727#eval542845; http://www.pnas.org/lookup/doi/10.1073/pnas.0704662104; http://www.pnas.org/content/104/29/11889; http://www.pnas.org/content/104/29/11889.abstract; http://www.pnas.org/content/104/29/11889.full.pdf; http://www.pnas.org/cgi/doi/10.1073/pnas.0704662104; https://www.pnas.org/content/104/29/11889.abstract; https://www.pnas.org/content/pnas/104/29/11889.full.pdf
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