The Use of 2,4-Dinitrophenylhydrazine Derivatives of Ovarian Ketosteroids in Steroid Analysis
Proceedings of the Iowa Academy of Science, Vol: 85, Issue: 3, Page: 99-102
1978
- 890Usage
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Usage890
- Downloads873
- Abstract Views17
Artifact Description
This paper reports the development of a technically simple analytical technique for the characterization and quantitative determination of ovarian ketosteroids employing derivative formation with 2,4-dinitrophenylhydrazine (2,4-DNP), separation by thin-layer chromatography (TLC), and quantitative determination by spectrophotometry. The chemical identities of the derivatives were established by nitrogen analysis. The melting point, chromatographic mobilities of the free steroids and their derivatives on silica gel G in three systems, wavelength of maximum absorption in chloroform and ethanol, and molar absorption coefficient are reported for the 2,4-DNP derivatives of ovarian ketosteroids. The relationship between the absorption maximum in chloroform and the chemical structure of the derivatives has been analyzed.
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