Understanding the gene regulatory patterns of H4Kac PTMs and their known BRD reader proteins ATAD2 and ATAD2B

Histone acetyllysine modifications are essential components of higher-order chromatin structure, and acetylation of five lysine residues on histone H4 (H4Kac) has been broadly associated with euchromatin and recruitment of regulatory factors in chromatin processes such as gene expression. The bromodomain-containing, AAA-ATPase proteins ATAD2 and ATAD2B have been identified to selectively recognize specific H4Kac modifications. Using CUT&RUN, we mapped the genomic enrichment sites for ATAD2, ATAD2B, and the H4Kac modifications. Through subsequent reduced dimensionality analysis, we interrogate distinct H4Kac modification patterns and correlate individual and combinatorial H4Kac regulatory patterns with the genomic enrichment of ATAD2 and ATAD2B.