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Extraction optimization of volatile oil from chinese elder herb by supercritical CO2 and its composition analysis

Vol: 33, Issue: 10, Page: 154-157
2017
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The extraction conditions of volatile oil from Chinese elder herb by supercritical CO2 were optimized by single factor experiment and response surface methodology. Flow rate of CO2, the pressure, temperature and time of extraction were employed to examine the influences of the yield of volatile oil. The chemical constituents were separated and identified by Gas chromatography-mass spectrometry (GC-MS). The optimal extracting conditions were flow rate of CO2 25 L/h, extraction pressure 30.5 MPa, at 45.0 ℃ for 2.8 h. The extraction rate of volatile oil from Chinese elder herb was 0.86%. 16 kinds of volatile compounds were separated from Chinese elder herb and accounted for 93.87% of the total extractions. The contents of the more compounds were 3-methylpentanoic acid (29.37%), 3-methylbutanoic acid (13.83%), 4-hexene-1-ol (8.27%), and 3-(ethylthio) butanoic acid (6.46%), respectively.

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