Cloning, expression and catalytic function analysis of chitinase from Streptomyces diastaticus
Food and Machinery, ISSN: 1003-5788, Vol: 38, Issue: 5, Page: 1-7
2022
- 1Citations
- 24Usage
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations1
- Citation Indexes1
- Usage24
- Downloads20
- Abstract Views4
Article Description
Objective; This study focused on improving the production of chitosan oligosaccharides hy chitinase degradation of chitin. Methods: Using genetic engineering methods to clone, prokaryotic expressing, and investigating enzymatic properties of the chitinase from Streptomyces diastaticus, and the chitinase activity was explored hy homology modeling, amino acid comparison of the catalytic domain, and site-directed mutagenesis. Results: After cloning and prokaryotic expression, the enzyme production cycle was shortened from 7 d to 24 h, and the enzyme activity reached 132 LJ/L, which was 32% higher than the original bacterial enzyme activity (100 U/L). The amino acids that determine the activity of chitinase was Asp at position 128, 130, and Glu at position 132 of the catalytic domain. Conclusion; Clonal expression of the chitinase gene resulted in a shorter enzyme production cycle and increased enzyme activity.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85201541923&origin=inward; http://dx.doi.org/10.13652/j.spjx.1003.5788.2022.90090; https://www.ifoodmm.cn/journal/vol38/iss5/1; https://www.ifoodmm.cn/cgi/viewcontent.cgi?article=1151&context=journal; https://dx.doi.org/10.13652/j.spjx.1003.5788.2022.90090; https://www.chndoi.org/Resolution/Handler?doi=10.13652/j.spjx.1003.5788.2022.90090
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