Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna
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Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed.