Ecological method development for detecting N-nitrosodimethylamine in water using HPLC-PDAD

Publication Year:
2010
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Repository URL:
https://commons.emich.edu/theses/260
Author(s):
McDay, JoLisa
Tags:
Dimethylnitrosamine Toxicity testing; Environmental testing; Alcohol; Extraction (Chemistry); Water Analysis Research; Chemistry
thesis / dissertation description
Using ethanol, a sustainable reversed-phase high-performance liquid chromatographic method with photo-diode array detection (HPLC-PDAD) was investigated to detect sub-parts per billion concentrations of N-nitrosodimethylamine (NDMA) in drinking water and environmental water samples. This presents a practical and “green” option to analytical methods that require laborious derivatization, expensive detectors and unecological solvents.Mobile phase characteristics, instrument operating parameters, and sample preparation were investigated. Selectivity for NDMA was evaluated using three different C18 columns. Optimal peak resolution and detection were achieved at 231 nm. The limit of detection and quantitation for NDMA was 0.29 μg/mL and 0.96 μg/mL, respectively, with an ethanol modifier and 5-μm column. A sustainable solid-phase extraction method was also investigated. Using ethanol for cartridge conditioning and extraction, recovery of NDMA was low (10%).