Protein Purification and Cation Exchange Protein Chromatography for Cytochrome C554 (number should be subscripted)

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Pellegrini, Frank
artifact description
Cytochrome C554 is a naturally occurring monomeric protein that has 4 c-type hemes. C554 is an electron transport protein that has been reported to also have the ability to reduce nitric oxide to nitrous oxide. The bacterium Nitrosomonas europaea uses C554 as part of its pathway for fixing ammonia to nitrite. C554 is being studied in Dr. Pacheco’s lab in conjunction with other proteins (HAO, ccNIR) that are also used in ammonia-nitrite interconversion by various bacteria. Being able to purify and optimize purification to obtain a high-yield of C554 is of high priority so that further studies can be done.