Salicylic acid activates sigma factor B by rsbU-dependent and -independent mechanisms.

Citation data:

Journal of bacteriology, ISSN: 0021-9193, Vol: 188, Issue: 16, Page: 5896-903

Publication Year:
2006
Usage 54
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Citations 12
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Repository URL:
https://digitalcommons.dartmouth.edu/facoa/1095
PMID:
16885458
DOI:
10.1128/jb.01960-05
PMCID:
PMC1540054
Author(s):
Palma, Marco; Bayer, Arnold; Kupferwasser, Leon I; Joska, Tammy; Yeaman, Michael R; Cheung, Ambrose
Publisher(s):
American Society for Microbiology
Tags:
Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology; antirheumatic agent; non steroidal antiinflammatory agent; analgesic; antipyretic; bacteriology; microbiology; mechanism; initiation factor σ; salicylic acid; antirhumatismal; Bacteriology; Medical Microbiology; Medical Sciences; Medicine and Health Sciences
article description
Salicylic acid (SAL) may impact Staphylococcus aureus virulence by activating the sigB operon (rsbU-V-W-sigB), thus leading to reductions in alpha-toxin production and decreased fibronectin binding (L. I. Kupferwasser et al., J. Clin. Investig. 112:222-233, 2003). As these prior studies were performed in strain RN6390 (an rsbU mutant) and its rsbU-repaired variant, SH1000, the current investigation was designed to determine if the SAL effect occurs via rsbU- and/or rsbV-dependent pathways in an rsbU-intact S. aureus strain (FDA486). We thus quantified the transcription from two sigB-dependent promoters (asp23 and sarA P3) in FDA486 in response to SAL exposure in vitro, using isogenic single-knockout constructs of rsbU, rsbV, or rsbW and a green fluorescent protein reporter system. SAL induced sarA P3 and asp23 promoter activities in a dose-dependent manner in the parental strain. In contrast, sigB activation by SAL was progressively more mitigated in the rsbU and rsbV mutants. As predicted, SAL caused significant reductions in both alpha-toxin production and fibrinogen and fibronectin binding in the parental strain. The extent of these reductions, compared with the parent, was reduced in the rsb mutants (rsbV > rsbU), especially at low SAL concentrations. Since generation of the free SigB protein usually requires a sequential rsbU-V-W-sigB activation cascade, the present phenotypic and genotypic data suggest key roles for both rsbU and rsbV in SAL-mediated activation of sigB in strains with a fully intact sigB operon.